2PWD

Crystal Structure of the Trehalulose Synthase MUTB from Pseudomonas Mesoacidophila MX-45 Complexed to the Inhibitor Deoxynojirmycin

2PWD の概要

• エントリーDOI: 10.2210/pdb2pwd/pdb
• 関連するPDBエントリー: 1ZJA 1ZJB
• 分子名称: Sucrose isomerase, CALCIUM ION, 1-DEOXYNOJIRIMYCIN, ... (4 entities in total)
• 機能のキーワード: trehalulose synthase, sucrose isomerase, alpha-amylase family, (beta/alpha)8 barrel, isomerase
• 由来する生物種: Pseudomonas mesoacidophila
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 128284.20

構造登録者

Ravaud, S.,Robert, X.,Haser, R.,Aghajari, N. (登録日: 2007-05-11, 公開日: 2007-06-26, 最終更新日: 2023-08-30)

主引用文献

Ravaud, S.,Robert, X.,Watzlawick, H.,Haser, R.,Mattes, R.,Aghajari, N.
Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization.
J.Biol.Chem., 61:100-103, 2007

PubMed Abstract: Various diseases related to the overconsumption of sugar make a growing need for sugar substitutes. Because sucrose is an inexpensive and readily available d-glucose donor, the industrial potential for enzymatic synthesis of the sucrose isomers trehalulose and/or isomaltulose from sucrose is large. The product specificity of sucrose isomerases that catalyze this reaction depends essentially on the possibility for tautomerization of sucrose, which is required for trehalulose formation. For optimal use of the enzyme, targeting controlled synthesis of these functional isomers, it is necessary to minimize the side reactions. This requires an extensive analysis of substrate binding modes and of the specificity-determining sites in the structure. The 1.6-2.2-A resolution three-dimensional structures of native and mutant complexes of a trehalulose synthase from Pseudomonas mesoacidophila MX-45 mimic successive states of the enzyme reaction. Combined with mutagenesis studies they give for the first time thorough insights into substrate recognition and processing and reaction specificities of these enzymes. Among the important outcomes of this study is the revelation of an aromatic clamp defined by Phe(256) and Phe(280) playing an essential role in substrate recognition and in controlling the reaction specificity, which is further supported by mutagenesis studies. Furthermore, this study highlights essential residues for binding the glucosyl and fructosyl moieties. The introduction of subtle changes informed by comparative three-dimensional structural data observed within our study can lead to fundamental modifications in the mode of action of sucrose isomerases and hence provide a template for industrial catalysts.

PubMed: 17597061
DOI: 10.1074/jbc.M704515200

実験手法

X-RAY DIFFRACTION (1.8 Å)