2PW0

crystal structure of trans-aconitate bound to methylaconitate isomerase PrpF from Shewanella oneidensis

2PW0 の概要

• エントリーDOI: 10.2210/pdb2pw0/pdb
• 関連するPDBエントリー: 2PVZ
• 分子名称: PrpF methylaconitate isomerase, 1,2-ETHANEDIOL, TRICARBALLYLIC ACID, ... (4 entities in total)
• 機能のキーワード: propionate catabolism, diaminopimelate epimerase like, aconitate binding, unknown function
• 由来する生物種: Shewanella oneidensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84129.46

構造登録者

Garvey, G.S.,Rayment, I.R. (登録日: 2007-05-10, 公開日: 2007-08-21, 最終更新日: 2024-02-21)

主引用文献

Garvey, G.S.,Rocco, C.J.,Escalante-Semerena, J.C.,Rayment, I.
The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans-aconitate: insights into its biological function.
Protein Sci., 16:1274-1284, 2007

PubMed Abstract: In bacteria, the dehydration of 2-methylcitrate to yield 2-methylaconitate in the 2-methylcitric acid cycle is catalyzed by a cofactor-less (PrpD) enzyme or by an aconitase-like (AcnD) enzyme. Bacteria that use AcnD also require the function of the PrpF protein, whose function was previously unknown. To gain insights into the function of PrpF, the three-dimensional crystal structure of the PrpF protein from the bacterium Shewanella oneidensis was solved at 2.0 A resolution. The protein fold of PrpF is strikingly similar to those of the non-PLP-dependent diaminopimelate epimerase from Haemophilus influenzae, a putative proline racemase from Brucella melitensis, and to a recently deposited structure of a hypothetical protein from Pseudomonas aeruginosa. Results from in vitro studies show that PrpF isomerizes trans-aconitate to cis-aconitate. It is proposed that PrpF catalysis of the cis-trans isomerization proceeds through a base-catalyzed proton abstraction coupled with a rotation about C2-C3 bond of 2-methylaconitate, and that residue Lys73 is critical for PrpF function. The newly identified function of PrpF as a non-PLP-dependent isomerase, together with the fact that PrpD-containing bacteria do not require PrpF, suggest that the isomer of 2-methylaconitate that serves as a substrate of aconitase must have the same stereochemistry as that synthesized by PrpD. From this, it follows that the 2-methylaconitate isomer generated by AcnD is not a substrate of aconitase, and that PrpF is required to generate the correct isomer. As a consequence, the isomerase activity of PrpF may now be viewed as an integral part of the 2-methylcitric acid cycle.

PubMed: 17567742
DOI: 10.1110/ps.072801907

実験手法

X-RAY DIFFRACTION (1.57 Å)