2PVX

NMR and X-ray Analysis of Structural Additivity in Metal Binding Site-Swapped Hybrids of Rubredoxin

2PVX の概要

• エントリーDOI: 10.2210/pdb2pvx/pdb
• 関連するPDBエントリー: 2PVE
• 分子名称: Rubredoxin, ZINC ION (3 entities in total)
• 機能のキーワード: rubredoxin, chimeric, pyrococcus furiosus, electron transport
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 49025.22

構造登録者

Wang, L.,LeMaster, D.M.,Hernandez, G.,Li, H. (登録日: 2007-05-10, 公開日: 2007-12-18, 最終更新日: 2023-08-30)

主引用文献

Lemaster, D.M.,Anderson, J.S.,Wang, L.,Guo, Y.,Li, H.,Hernandez, G.
NMR and X-ray analysis of structural additivity in metal binding site-swapped hybrids of rubredoxin
Bmc Struct.Biol., 7:81-81, 2007

PubMed Abstract: Chimeric hybrids derived from the rubredoxins of Pyrococcus furiosus (Pf) and Clostridium pasteurianum (Cp) provide a robust system for the characterization of protein conformational stability and dynamics in a differential mode. Interchange of the seven nonconserved residues of the metal binding site between the Pf and Cp rubredoxins yields a complementary pair of hybrids, for which the sum of the thermodynamic stabilities is equal to the sum for the parental proteins. Furthermore, the increase in amide hydrogen exchange rates for the hyperthermophile-derived metal binding site hybrid is faithfully mirrored by a corresponding decrease for the complementary hybrid that is derived from the less thermostable rubredoxin, indicating a degree of additivity in the conformational fluctuations that underlie these exchange reactions.

PubMed: 18053245
DOI: 10.1186/1472-6807-7-81

実験手法

X-RAY DIFFRACTION (1.04 Å)