2PV6

HIV-1 gp41 Membrane Proximal Ectodomain Region peptide in DPC micelle

2PV6 の概要

• エントリーDOI: 10.2210/pdb2pv6/pdb
• 分子名称: Envelope glycoprotein (1 entity in total)
• 機能のキーワード: kinked helix, viral protein
• 由来する生物種: Human immunodeficiency virus 1
• 細胞内の位置: Transmembrane protein gp41: Virion membrane; Single-pass type I membrane protein. Surface protein gp120: Virion membrane; Peripheral membrane protein: P04578
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2929.33

構造登録者

Sun, Z.-Y.J.,Oh, K.J.,Kim, M.,Reinherz, E.L.,Wagner, G. (登録日: 2007-05-09, 公開日: 2008-03-18, 最終更新日: 2024-05-22)

主引用文献

Sun, Z.Y.,Oh, K.J.,Kim, M.,Yu, J.,Brusic, V.,Song, L.,Qiao, Z.,Wang, J.H.,Wagner, G.,Reinherz, E.L.
HIV-1 broadly neutralizing antibody extracts its epitope from a kinked gp41 ectodomain region on the viral membrane
Immunity, 28:52-63, 2008

PubMed Abstract: Although rarely elicited during natural human infection, the most broadly neutralizing antibodies (BNAbs) against diverse human immunodeficiency virus (HIV)-1 strains target the membrane-proximal ectodomain region (MPER) of viral gp41. To gain insight into MPER antigenicity, immunogenicity, and viral function, we studied its structure in the lipid environment by a combination of nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), and surface plasmon resonance (SPR) techniques. The analyses revealed a tilted N-terminal alpha helix (aa 664-672) connected via a short hinge to a flat C-terminal helical segment (675-683). This metastable L-shaped structure is immersed in viral membrane and, therefore, less accessible to immune attack. Nonetheless, the 4E10 BNAb extracts buried W672 and F673 after initial encounter with the surface-embedded MPER. The data suggest how BNAbs may perturb tryptophan residue-associated viral fusion involving the mobile N-terminal MPER segment and, given conservation of MPER sequences in HIV-1, HIV-2, and SIV, have important implications for structure-guided vaccine design.

PubMed: 18191596
DOI: 10.1016/j.immuni.2007.11.018

実験手法

SOLUTION NMR