2PQU

Crystal structure of KH1 domain of human PCBP2 complexed to single-stranded 12-mer telomeric dna

Summary for 2PQU

• Entry DOI: 10.2210/pdb2pqu/pdb
• Related: 2AXY
• Descriptor: 12-mer C-rich strand of human telomeric DNA, Poly(rC)-binding protein 2 (3 entities in total)
• Functional Keywords: dna binding protein-dna complex, rna and dna binding protein-dna complex, rna and dna binding protein/dna
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 6
• Total formula weight: 39767.09

Authors

James, T.L.,Lee, J. (deposition date: 2007-05-02, release date: 2007-06-12, Last modification date: 2024-11-06)

Primary citation

Du, Z.,Lee, J.K.,Fenn, S.,Tjhen, R.,Stroud, R.M.,James, T.L.
X-ray crystallographic and NMR studies of protein-protein and protein-nucleic acid interactions involving the KH domains from human poly(C)-binding protein-2.
Rna, 13:1043-1051, 2007

PubMed Abstract: Poly(C)-binding proteins (PCBPs) are KH (hnRNP K homology) domain-containing proteins that recognize poly(C) DNA and RNA sequences in mammalian cells. Binding poly(C) sequences via the KH domains is critical for PCBP functions. To reveal the mechanisms of KH domain-D/RNA recognition and its functional importance, we have determined the crystal structures of PCBP2 KH1 domain in complex with a 12-nucleotide DNA corresponding to two repeats of the human C-rich strand telomeric DNA and its RNA equivalent. The crystal structures reveal molecular details for not only KH1-DNA/RNA interaction but also protein-protein interaction between two KH1 domains. NMR studies on a protein construct containing two KH domains (KH1 + KH2) of PCBP2 indicate that KH1 interacts with KH2 in a way similar to the KH1-KH1 interaction. The crystal structures and NMR data suggest possible ways by which binding certain nucleic acid targets containing tandem poly(C) motifs may induce structural rearrangement of the KH domains in PCBPs; such structural rearrangement may be crucial for some PCBP functions.

PubMed: 17526645
DOI: 10.1261/rna.410107

Experimental method

X-RAY DIFFRACTION (2.12 Å)