2PP4

Solution Structure of ETO-TAFH refined in explicit solvent

2PP4 の概要

• エントリーDOI: 10.2210/pdb2pp4/pdb
• NMR情報: BMRB: 7396
• 分子名称: Protein ETO (1 entity in total)
• 機能のキーワード: transcriptional cofactor, leukemia, 4-helix bundle, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Potential): Q06455
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12137.99

構造登録者

Wei, Y.,Liu, S.,Lausen, J.,Woodrell, C.,Cho, S.,Biris, N.,Kobayashi, N.,Yokoyama, S.,Werner, M.H. (登録日: 2007-04-27, 公開日: 2007-06-19, 最終更新日: 2024-05-22)

主引用文献

Wei, Y.,Liu, S.,Lausen, J.,Woodrell, C.,Cho, S.,Biris, N.,Kobayashi, N.,Wei, Y.,Yokoyama, S.,Werner, M.H.
A TAF4-homology domain from the corepressor ETO is a docking platform for positive and negative regulators of transcription
Nat.Struct.Mol.Biol., 14:653-661, 2007

PubMed Abstract: The eight twenty-one protein, ETO, is implicated in 12%-15% of acute human leukemias as part of a gene fusion with RUNX1 (also called AML1). Of the four ETO domains related to Drosophila melanogaster Nervy, only two are required to induce spontaneous myeloid leukemia upon transplantation into the mouse. One of these domains is related in sequence to TAF4, a component of TFIID. The structure of this domain, ETO-TAFH, is similar to yeast Rpb4 and to Escherichia coli sigma(70); it is the first TAF-related protein with structural similarity to the multisubunit RNA polymerases. Overlapping surfaces of ETO-TAFH interact with an autonomous repression domain of the nuclear receptor corepressor N-CoR and with a conserved activation domain from the E-box family of transcription factors. Thus, ETO-TAFH acts as a structural platform that can interchange negative and positive coregulatory proteins to control transcription.

PubMed: 17572682
DOI: 10.1038/nsmb1258

実験手法

SOLUTION NMR