2PMZ

Archaeal RNA polymerase from Sulfolobus solfataricus

Summary for 2PMZ

• Entry DOI: 10.2210/pdb2pmz/pdb
• Descriptor: DNA-directed RNA polymerase subunit A, DNA-directed RNA polymerase subunit N, DNA-directed RNA polymerase subunit P, ... (14 entities in total)
• Functional Keywords: 4fe-4s cluster binding motif, translation, transferase
• Biological source: Sulfolobus solfataricus; More
• Total number of polymer chains: 22
• Total formula weight: 756488.97

Authors

Murakami, K.S. (deposition date: 2007-04-23, release date: 2008-02-12, Last modification date: 2024-11-20)

Primary citation

Hirata, A.,Klein, B.J.,Murakami, K.S.
The X-ray crystal structure of RNA polymerase from Archaea
Nature, 451:851-854, 2008

PubMed Abstract: The transcription apparatus in Archaea can be described as a simplified version of its eukaryotic RNA polymerase (RNAP) II counterpart, comprising an RNAPII-like enzyme as well as two general transcription factors, the TATA-binding protein (TBP) and the eukaryotic TFIIB orthologue TFB. It has been widely understood that precise comparisons of cellular RNAP crystal structures could reveal structural elements common to all enzymes and that these insights would be useful in analysing components of each enzyme that enable it to perform domain-specific gene expression. However, the structure of archaeal RNAP has been limited to individual subunits. Here we report the first crystal structure of the archaeal RNAP from Sulfolobus solfataricus at 3.4 A resolution, completing the suite of multi-subunit RNAP structures from all three domains of life. We also report the high-resolution (at 1.76 A) crystal structure of the D/L subcomplex of archaeal RNAP and provide the first experimental evidence of any RNAP possessing an iron-sulphur (Fe-S) cluster, which may play a structural role in a key subunit of RNAP assembly. The striking structural similarity between archaeal RNAP and eukaryotic RNAPII highlights the simpler archaeal RNAP as an ideal model system for dissecting the molecular basis of eukaryotic transcription.

PubMed: 18235446
DOI: 10.1038/nature06530

Experimental method

X-RAY DIFFRACTION (3.4 Å)