2PD8

1.8 Angstrom Crystal Structure of the Cys71Ser mutant of Vivid

2PD8 の概要

• エントリーDOI: 10.2210/pdb2pd8/pdb
• 関連するPDBエントリー: 2PD7 2PDR 2PDT
• 分子名称: Vivid PAS protein VVD, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: lov domain; flavin; photoreceptor; circadian clock, circadian clock protein
• 由来する生物種: Neurospora crassa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35539.99

構造登録者

Zoltowski, B.D.,Crane, B.R.,Bilwes, A.M. (登録日: 2007-03-31, 公開日: 2007-06-05, 最終更新日: 2023-08-30)

主引用文献

Zoltowski, B.D.,Schwerdtfeger, C.,Widom, J.,Loros, J.J.,Bilwes, A.M.,Dunlap, J.C.,Crane, B.R.
Conformational switching in the fungal light sensor Vivid
Science, 316:1054-1057, 2007

PubMed Abstract: The Neurospora crassa photoreceptor Vivid tunes blue-light responses and modulates gating of the circadian clock. Crystal structures of dark-state and light-state Vivid reveal a light, oxygen, or voltage Per-Arnt-Sim domain with an unusual N-terminal cap region and a loop insertion that accommodates the flavin cofactor. Photoinduced formation of a cystein-flavin adduct drives flavin protonation to induce an N-terminal conformational change. A cysteine-to-serine substitution remote from the flavin adenine dinucleotide binding site decouples conformational switching from the flavin photocycle and prevents Vivid from sending signals in Neurospora. Key elements of this activation mechanism are conserved by other photosensors such as White Collar-1, ZEITLUPE, ENVOY, and flavin-binding, kelch repeat, F-BOX 1 (FKF1).

PubMed: 17510367
DOI: 10.1126/science.1137128

実験手法

X-RAY DIFFRACTION (1.8 Å)