2P6S

Crystal Structure of Transcriptional Regulator NMB0573/L-Met Complex from Neisseria Meningitidis

2P6S の概要

• エントリーDOI: 10.2210/pdb2p6s/pdb
• 分子名称: Transcriptional regulator, LRP/AsnC family, METHIONINE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: nmb0573, transcriptional regulator, lrp/asnc-family, structural genomics, structural and functional analysis of n. meninigitidis transcriptional regulators, oxford protein production facility, oppf, transcription
• 由来する生物種: Neisseria meningitidis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 148969.13

構造登録者

Ren, J.,Sainsbury, S.,Owens, R.J.,Oxford Protein Production Facility (OPPF) (登録日: 2007-03-19, 公開日: 2007-04-03, 最終更新日: 2024-10-16)

主引用文献

Ren, J.,Sainsbury, S.,Combs, S.E.,Capper, R.G.,Jordan, P.W.,Berrow, N.S.,Stammers, D.K.,Saunders, N.J.,Owens, R.J.
The Structure and Transcriptional Analysis of a Global Regulator from Neisseria meningitidis.
J.Biol.Chem., 282:14655-14664, 2007

PubMed Abstract: Neisseria meningitidis, a causative agent of bacterial meningitis, has a relatively small repertoire of transcription factors, including NMB0573 (annotated AsnC), a member of the Lrp-AsnC family of regulators that are widely expressed in both Bacteria and Archaea. In the present study we show that NMB0573 binds to l-leucine and l-methionine and have solved the structure of the protein with and without bound amino acids. This has shown, for the first time that amino acid binding does not induce significant conformational changes in the structure of an AsnC/Lrp regulator although it does appear to stabilize the octameric assembly of the protein. Transcriptional profiling of wild-type and NMB0573 knock-out strains of N. meningitidis has shown that NMB0573 is associated with an adaptive response to nutrient poor conditions reflected in a reduction in major surface protein expression. On the basis of its structure and the transcriptional response, we propose that NMB0573 is a global regulator in Neisseria controlling responses to nutrient availability through indicators of general amino acid abundance: leucine and methionine.

PubMed: 17374605
DOI: 10.1074/jbc.M701082200

実験手法

X-RAY DIFFRACTION (2.8 Å)