2P4W

Crystal structure of heat shock regulator from Pyrococcus furiosus

「1XNP」から置き換えられました

2P4W の概要

• エントリーDOI: 10.2210/pdb2p4w/pdb
• 関連するPDBエントリー: 1XNP
• 分子名称: Transcriptional regulatory protein arsR family, SULFATE ION (3 entities in total)
• 機能のキーワード: archaea, phr, heat shock, transcriptional regulation, winged helix, dna binding, transcription
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48244.25

構造登録者

Liu, W.,Vierke, G.,Panjikar, S.,Thomm, M.,Ladenstein, R. (登録日: 2007-03-13, 公開日: 2007-03-27, 最終更新日: 2023-08-30)

主引用文献

Liu, W.,Vierke, G.,Wenke, A.K.,Thomm, M.,Ladenstein, R.
Crystal Structure of the Archaeal Heat Shock Regulator from Pyrococcus furiosus: A Molecular Chimera Representing Eukaryal and Bacterial Features.
J.Mol.Biol., 369:474-488, 2007

PubMed Abstract: We report here the crystal structure of a protein from Pyrococcus furiosus (Phr) that represents the first characterized heat shock transcription factor in archaea. Phr specifically represses the expression of heat shock genes at physiological temperature in vitro and in vivo but is released from the promoters upon heat shock response. Structure analysis revealed a stable homodimer, each subunit consisting of an N-terminal winged helix DNA-binding domain (wH-DBD) and a C-terminal antiparallel coiled coil helical domain. The overall structure shows as a molecular chimera with significant folding similarity of its DBD to the bacterial SmtB/ArsR family, while its C-terminal part was found to be a remote homologue of the eukaryotic BAG domain. The dimeric protein recognizes a palindromic DNA sequence. Molecular docking and mutational analyses suggested a novel binding mode in which the major specific contacts occur at the minor groove interacting with the strongly basic wing containing a cluster of three arginine residues.

PubMed: 17434531
DOI: 10.1016/j.jmb.2007.03.044

実験手法

X-RAY DIFFRACTION (2.6 Å)