2P45
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.1A resolution: SE5B-ORTHO-1 crystal form with five se-met sites (L4M, M34, M51, F68M, M83) in vhh scaffold.
Summary for 2P45
Entry DOI | 10.2210/pdb2p45/pdb |
Descriptor | Ribonuclease pancreatic, ANTIBODY CAB-RN05, SULFATE ION, ... (4 entities in total) |
Functional Keywords | semet phasing, camelid single-domain antibody, vhh, cab-rn05, rnase a, yeast surface display, hydrolase-immune system complex, hydrolase/immune system |
Biological source | Camelus dromedarius (Arabian camel) More |
Cellular location | Secreted: P61823 |
Total number of polymer chains | 2 |
Total formula weight | 27241.45 |
Authors | Tereshko, V.,Uysal, S.,Koide, A.,Margalef, K.,Koide, S.,Kossiakoff, A.A. (deposition date: 2007-03-11, release date: 2008-03-11, Last modification date: 2011-07-13) |
Primary citation | Tereshko, V.,Uysal, S.,Koide, A.,Margalef, K.,Koide, S.,Kossiakoff, A.A. Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold Protein Sci., 17:1175-1187, 2008 Cited by PubMed: 18445622DOI: 10.1110/ps.034892.108 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.1 Å) |
Structure validation
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