2P42

Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.8A resolution: SE3-mono-2 crystal form with three se-met sites (M34, M51, M83) in vhh scaffold

2P42 の概要

• エントリーDOI: 10.2210/pdb2p42/pdb
• 分子名称: Ribonuclease pancreatic, ANTIBODY CAB-RN05, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: semet phasing, camelid single-domain antibody, vhh, cab-rn05, rnase a, yeast surface display., hydrolase-immune system complex, hydrolase/immune system
• 由来する生物種: Camelus dromedarius (Arabian camel); 詳細
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53931.26

構造登録者

Tereshko, V.,Uysal, S.,Koide, A.,Margalef, K.,Koide, S.,Kossiakoff, A.A. (登録日: 2007-03-11, 公開日: 2008-03-11, 最終更新日: 2011-07-13)

• 主引用文献: Tereshko, V.,Uysal, S.,Koide, A.,Margalef, K.,Koide, S.,Kossiakoff, A.A.; Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold; Protein Sci., 17:1175-1187, 2008; PubMed: 18445622; DOI: 10.1110/ps.034892.108

実験手法

X-RAY DIFFRACTION (1.8 Å)