2P0B

Crystal structure of chemically-reduced E.coli nrfB

2P0B の概要

• エントリーDOI: 10.2210/pdb2p0b/pdb
• 関連するPDBエントリー: 2OZY
• 分子名称: Cytochrome c-type protein nrfB, HEME C (3 entities in total)
• 機能のキーワード: pentaheme c-type cytochrome, electron transport
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm (Potential): P0ABL1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21278.98

構造登録者

Clarke, T.A.,Richardson, D.J.,Hemmings, A.M. (登録日: 2007-02-28, 公開日: 2007-08-07, 最終更新日: 2024-10-30)

主引用文献

Clarke, T.A.,Cole, J.A.,Richardson, D.J.,Hemmings, A.M.
The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA.
Biochem.J., 406:19-30, 2007

PubMed Abstract: NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 A (1 A=0.1 nm) resolution. The architecture of the protein is that of a 40 A 'nanowire' in which the five haems are positioned within 6 A of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.

PubMed: 17521287
DOI: 10.1042/BJ20070321

実験手法

X-RAY DIFFRACTION (1.74 Å)