2OYW

Neurotensin in TFE:H2O (80:20)

2OYW の概要

• エントリーDOI: 10.2210/pdb2oyw/pdb
• 関連するPDBエントリー: 2OYV
• NMR情報: BMRB: 15145
• 分子名称: neurotensin (1 entity in total)
• 機能のキーワード: extended structure, neuropeptide
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1675.95

構造登録者

Monti, J.P.,Coutant, J.,Curmi, P.A. (登録日: 2007-02-23, 公開日: 2007-05-08, 最終更新日: 2024-11-20)

主引用文献

Coutant, J.,Curmi, P.A.,Toma, F.,Monti, J.P.
NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition.
Biochemistry, 46:5656-5663, 2007

PubMed Abstract: Neurotensin (NT) is a 13-residue neuropeptide that exerts multiple biological functions in the central and peripheral nervous system. Little is known about the structure of this neuropeptide, and what is known only concerns its C-terminal part. We determined here for the first time the structure of the full-length NT in membrane-mimicking environments by means of classical proton-proton distance constraints derived from solution-state NMR spectroscopy. NT was found to have a structure at both its N and C termini, whereas the central region of NT remains highly flexible. In TFE and HFIP solutions, the NT C-terminus presents an extended slightly incurved structure, whereas in DPC it has a beta turn. The N-terminal region of NT possesses great adaptability and accessibility to the microenvironment in the three media studied. Altogether, our work demonstrates a structure of NT fully compatible with its NTR-bound state.

PubMed: 17441729
DOI: 10.1021/bi602567p

実験手法

SOLUTION NMR