2OXL

Structure and Function of the E. coli Protein YmgB: a Protein Critical for Biofilm Formation and Acid Resistance

2OXL の概要

• エントリーDOI: 10.2210/pdb2oxl/pdb
• 分子名称: Hypothetical protein ymgB, octyl beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: bacterial protein, biofilm, acid resistance, dna binding protein, dimer, gene regulation
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14924.45

構造登録者

Page, R.,Peti, W.,Woods, T.K.,Palermino, J.M.,Doshi, O. (登録日: 2007-02-20, 公開日: 2007-10-30, 最終更新日: 2024-10-30)

主引用文献

Lee, J.,Page, R.,Garcia-Contreras, R.,Palermino, J.M.,Zhang, X.S.,Doshi, O.,Wood, T.K.,Peti, W.
Structure and Function of the Escherichia coli Protein YmgB: A Protein Critical for Biofilm Formation and Acid-resistance.
J.Mol.Biol., 373:11-26, 2007

PubMed Abstract: The Escherichia coli gene cluster ymgABC was identified in transcriptome studies to have a role in biofilm development and stability. In this study, we showed that YmgB represses biofilm formation in rich medium containing glucose, decreases cellular motility, and protects the cell from acid indicating that YmgB has a major role in acid-resistance in E. coli. Our data show that these phenotypes are potentially mediated through interactions with the important cell signal indole. In addition, gel mobility-shift assays suggest that YmgB may be a non-specific DNA-binding protein. Using nickel-enrichment DNA microarrays, we showed that YmgB binds, either directly or indirectly, via a probable ligand, genes important for biofilm formation. To advance our understanding of the function of YmgB, we used X-ray crystallography to solve the structure of the protein to 1.8 A resolution. YmgB is a biological dimer that is structurally homologous to the E. coli gene regulatory protein Hha, despite having only 5% sequence identity. This supports our DNA microarray data showing that YmgB is a gene regulatory protein. Therefore, this protein, which clearly has a critical role in acid-resistance in E. coli, has been renamed as AriR for regulator of acid resistance influenced by indole.

PubMed: 17765265
DOI: 10.1016/j.jmb.2007.07.037

実験手法

X-RAY DIFFRACTION (1.8 Å)