2OXG

The SoxYZ Complex of Paracoccus pantotrophus

Summary for 2OXG

• Entry DOI: 10.2210/pdb2oxg/pdb
• Related: 2OX5
• Descriptor: SoxZ protein, SoxY protein, SULFATE ION, ... (5 entities in total)
• Functional Keywords: immunoglobulin-like beta-sandwich fold, transport protein
• Biological source: Paracoccus denitrificans; More
• Total number of polymer chains: 8
• Total formula weight: 97032.76

Authors

Sauve, V.,Berks, B.C.,Hemmings, A.M. (deposition date: 2007-02-20, release date: 2007-05-22, Last modification date: 2023-08-30)

Primary citation

Sauve, V.,Bruno, S.,Berks, B.C.,Hemmings, A.M.
The SoxYZ Complex Carries Sulfur Cycle Intermediates on a Peptide Swinging Arm.
J.Biol.Chem., 282:23194-23204, 2007

PubMed Abstract: The bacterial Sox (sulfur oxidizing) system allows the utilization of inorganic sulfur compounds in energy metabolism. Central to this process is the SoxYZ complex that carries the pathway intermediates on a cysteine residue near the C terminus of SoxY. Crystal structures have been determined for Paracoccus pantotrophus SoxYZ with the carrier cysteine in the underivatized state, conjugated to the polysulfide mimic beta-mercaptoethanol, and as the sulfonate adduct pathway intermediate. The carrier cysteine is located on a peptide swinging arm and is bracketed on either side by diglycine dipeptides acting as molecular universal joints. This structure provides a novel solution to the requirement that the cysteine-bound intermediates be able to access and orient themselves within the active sites of multiple partner enzymes. Adjacent to the swinging arm there is a conserved, deep, apolar pocket into which the beta-mercaptoethanol adduct extends. This pocket would be well suited to a role in protecting labile pathway intermediates from adventitious reactions.

PubMed: 17522046
DOI: 10.1074/jbc.M701602200

Experimental method

X-RAY DIFFRACTION (1.4 Å)