2OVS

Crystal structure of a Type Three secretion System protein

2OVS の概要

• エントリーDOI: 10.2210/pdb2ovs/pdb
• 分子名称: L0044, TETRAETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: global regulator protein type three secretion system, gene regulation, ligand binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27140.08

構造登録者

Jobichen, C.,Li, M.,Sivaraman, J. (登録日: 2007-02-15, 公開日: 2007-05-29, 最終更新日: 2024-10-30)

主引用文献

Jobichen, C.,Li, M.,Yerushalmi, G.,Tan, Y.W.,Mok, Y.K.,Rosenshine, I.,Leung, K.Y.,Sivaraman, J.
Structure of GrlR and the implication of its EDED motif in mediating the regulation of type III secretion system in EHEC.
Plos Pathog., 3:e69-e69, 2007

PubMed Abstract: Enterohemorrhagic Escherichia coli (EHEC) is a common cause of severe hemorrhagic colitis. EHEC's virulence is dependent upon a type III secretion system (TTSS) encoded by 41 genes. These genes are organized in several operons clustered in the locus of enterocyte effacement. Most of the locus of enterocyte effacement genes, including grlA and grlR, are positively regulated by Ler, and Ler expression is positively and negatively modulated by GrlA and GrlR, respectively. However, the molecular basis for the GrlA and GrlR activity is still elusive. We have determined the crystal structure of GrlR at 1.9 A resolution. It consists of a typical beta-barrel fold with eight beta-strands containing an internal hydrophobic cavity and a plug-like loop on one side of the barrel. Strong hydrophobic interactions between the two beta-barrels maintain the dimeric architecture of GrlR. Furthermore, a unique surface-exposed EDED (Glu-Asp-Glu-Asp) motif is identified to be critical for GrlA-GrlR interaction and for the repressive activity of GrlR. This study contributes a novel molecular insight into the mechanism of GrlR function.

PubMed: 17511515
DOI: 10.1371/journal.ppat.0030069

実験手法

X-RAY DIFFRACTION (1.9 Å)