2OV6

The NMR structure of subunit F of the Methanogenic A1Ao ATP synthase and its interaction with the nucleotide-binding subunit B

2OV6 の概要

• エントリーDOI: 10.2210/pdb2ov6/pdb
• NMR情報: BMRB: 15046
• 分子名称: V-type ATP synthase subunit F (1 entity in total)
• 機能のキーワード: f subunit, a1ao atp synthase, hydrolase
• 由来する生物種: Methanosarcina mazei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10777.30

構造登録者

Gayen, S.,Subramanian, V.,Biukovic, G. (登録日: 2007-02-13, 公開日: 2007-12-25, 最終更新日: 2024-05-15)

主引用文献

Gayen, S.,Vivekanandan, S.,Biukovic, G.,Gruber, G.,Yoon, H.S.
NMR solution structure of subunit F of the methanogenic A1AO adenosine triphosphate synthase and its interaction with the nucleotide-binding subunit B.
Biochemistry, 46:11684-11694, 2007

PubMed Abstract: The A1AO adenosine triphosphate (ATP) synthase from archaea uses the ion gradients generated across the membrane sector (AO) to synthesize ATP in the A3B3 domain of the A1 sector. The energy coupling between the two active domains occurs via the so-called stalk part(s), to which the 12 kDa subunit F does belong. Here, we present the solution structure of the F subunit of the A1AO ATP synthase from Methanosarcina mazei Gö1. Subunit F exhibits a distinct two-domain structure, with the N-terminal having 78 residues and residues 79-101 forming the flexible C-terminal part. The well-ordered N-terminal domain is composed of a four-stranded parallel beta-sheet structure and three alpha-helices placed alternately. The two domains are loosely associated with more flexibility relative to each other. The flexibility of the C-terminal domain is further confirmed by dynamics studies. In addition, the affinity of binding of mutant subunit F, with a substitution of Trp100 against Tyr and Ile at the very C-terminal end, to the nucleotide-binding subunit B was determined quantitatively using the fluorescence signals of natural subunit B (Trp430). Finally, the arrangement of subunit F within the complex is presented.

PubMed: 17910473
DOI: 10.1021/bi701102n

実験手法

SOLUTION NMR