2OQO

Crystal structure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis

2OQO の概要

• エントリーDOI: 10.2210/pdb2oqo/pdb
• 分子名称: Penicillin-binding protein 1A (PBP-1a) (PBP1a), 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, 3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE, ... (4 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cell inner membrane ; Single- pass type II membrane protein : O66874
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23765.61

構造登録者

Yuan, Y.,Sliz, P.,Walker, S. (登録日: 2007-01-31, 公開日: 2007-03-13, 最終更新日: 2023-12-27)

主引用文献

Yuan, Y.,Barrett, D.,Zhang, Y.,Kahne, D.,Sliz, P.,Walker, S.
Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis.
Proc.Natl.Acad.Sci.Usa, 104:5348-5353, 2007

PubMed Abstract: Peptidoglycan is an essential polymer that forms a protective shell around bacterial cell membranes. Peptidoglycan biosynthesis is the target of many clinically used antibiotics, including the beta-lactams, imipenems, cephalosporins, and glycopeptides. Resistance to these and other antibiotics has prompted interest in an atomic-level understanding of the enzymes that make peptidoglycan. Representative structures have been reported for most of the enzymes in the pathway. Until now, however, there have been no structures of any peptidoglycan glycosyltransferases (also known as transglycosylases), which catalyze formation of the carbohydrate chains of peptidoglycan from disaccharide subunits on the bacterial cell surface. We report here the 2.1-A crystal structure of the peptidoglycan glycosyltransferase (PGT) domain of Aquifex aeolicus PBP1A. The structure has a different fold from all other glycosyltransferase structures reported to date, but it bears some resemblance to lambda-lysozyme, an enzyme that degrades the carbohydrate chains of peptidoglycan. An analysis of the structure, combined with biochemical information showing that these enzymes are processive, suggests a model for glycan chain polymerization.

PubMed: 17360321
DOI: 10.1073/pnas.0701160104

実験手法

X-RAY DIFFRACTION (2.1 Å)