2OQ9

Structure of the non-canonical Mcol5 of Hydra nematocysts

2OQ9 の概要

• エントリーDOI: 10.2210/pdb2oq9/pdb
• 分子名称: Minicollagen-5 (1 entity in total)
• 機能のキーワード: disulfide, cis-proline, nematocyst, non-canonical crd, cysteine-rich domain, structural protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2363.82

構造登録者

Meier, S.,Oezbek, S.,Grzesiek, S. (登録日: 2007-01-31, 公開日: 2007-12-18, 最終更新日: 2024-11-20)

主引用文献

Adamczyk, P.,Meier, S.,Gross, T.,Hobmayer, B.,Grzesiek, S.,Bachinger, H.P.,Holstein, T.W.,Ozbek, S.
Minicollagen-15, a novel minicollagen isolated from Hydra, forms tubule structures in nematocysts.
J.Mol.Biol., 376:1008-1020, 2008

PubMed Abstract: Minicollagens constitute a family of unusually short collagen molecules isolated from cnidarians. They are restricted to the nematocyst, a cylindrical explosive organelle serving in defense and capture of prey. The nematocyst capsule contains a long tubule inside of its matrix, which is expelled and everted during an ultrafast discharge process. Here, we report the cloning and characterization of a novel minicollagen in Hydra, designated minicollagen-15 (NCol-15). NCol-15, like NCol-3 and NCol-4, shows deviations from the canonical cysteine pattern in its terminal cysteine-rich domains (CRDs). Minicollagens share common domain architectures with a central collagen sequence flanked by polyproline stretches and short N- and C-terminal CRDs. The CRDs are involved in the formation of a highly resistant cysteine network, which constitutes the basic structure of the nematocyst capsule. Unlike NCol-1, which is part of the capsule wall, NCol-15 is localized to tubules, arguing for a functional differentiation of minicollagens within the nematocyst architecture. NMR analysis of the altered C-terminal CRD of NCol-15 showed a novel disulfide-linked structure within the cysteine-containing region exhibiting similar folding kinetics and stability as the canonical CRDs. Our data provide evidence for evolutionary diversification among minicollagens, which probably facilitated alterations in the morphology of the nematocyst wall and tubule.

PubMed: 18206162
DOI: 10.1016/j.jmb.2007.10.090

実験手法

SOLUTION NMR