2OOY

Crystal structure of the adenylate sensor from AMP-activated protein kinase complexed with ATP

2OOY の概要

• エントリーDOI: 10.2210/pdb2ooy/pdb
• 関連するPDBエントリー: 2OOX
• 分子名称: SNF1-like protein kinase ssp2, SPCC1919.03c protein, Hypothetical protein C1556.08c in chromosome I, ... (6 entities in total)
• 機能のキーワード: ampk, kinase, amp, transferase
• 由来する生物種: Schizosaccharomyces pombe (fission yeast); 詳細
• 細胞内の位置: Cytoplasm: P78789
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 129470.96

構造登録者

Townley, R.,Shapiro, L. (登録日: 2007-01-26, 公開日: 2007-02-06, 最終更新日: 2023-08-30)

主引用文献

Townley, R.,Shapiro, L.
Crystal structures of the adenylate sensor from fission yeast AMP-activated protein kinase.
Science, 315:1726-1729, 2007

PubMed Abstract: The 5'-AMP (adenosine monophosphate)-activated protein kinase (AMPK) coordinates metabolic function with energy availability by responding to changes in intracellular ATP (adenosine triphosphate) and AMP concentrations. Here, we report crystal structures at 2.9 and 2.6 A resolution for ATP- and AMP-bound forms of a core alphabetagamma adenylate-binding domain from the fission yeast AMPK homolog. ATP and AMP bind competitively to a single site in the gamma subunit, with their respective phosphate groups positioned near function-impairing mutants. Unexpectedly, ATP binds without counterions, amplifying its electrostatic effects on a critical regulatory region where all three subunits converge.

PubMed: 17289942
DOI: 10.1126/science.1137503

実験手法

X-RAY DIFFRACTION (2.88 Å)