2OIX

Xanthomonas XopD C470A Mutant

2OIX の概要

• エントリーDOI: 10.2210/pdb2oix/pdb
• 関連するPDBエントリー: 2OIV
• 分子名称: Xanthomonas outer protein D (2 entities in total)
• 機能のキーワード: clan ce family 48 cysteine protease, type iii secreted effector, desumoylating enzyme, secreted virulence facter, peptidase activity, isopeptidase activity, hydrolase
• 由来する生物種: Xanthomonas euvesicatoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20731.05

構造登録者

Chosed, R.,Tomchick, D.R.,Brautigam, C.A.,Machius, M.,Orth, K. (登録日: 2007-01-11, 公開日: 2007-05-29, 最終更新日: 2023-08-30)

主引用文献

Chosed, R.,Tomchick, D.R.,Brautigam, C.A.,Mukherjee, S.,Negi, V.S.,Machius, M.,Orth, K.
Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases.
J.Biol.Chem., 282:6773-6782, 2007

PubMed Abstract: XopD (Xanthomonas outer protein D), a type III secreted effector from Xanthomonas campestris pv. vesicatoria, is a desumoylating enzyme with strict specificity for its plant small ubiquitin-like modifier (SUMO) substrates. Based on SUMO sequence alignments and peptidase assays with various plant, yeast, and mammalian SUMOs, we identified residues in SUMO that contribute to XopD/SUMO recognition. Further predictions regarding the enzyme/substrate specificity were made by solving the XopD crystal structure. By incorporating structural information with sequence alignments and enzyme assays, we were able to elucidate determinants of the rigid SUMO specificity exhibited by the Xanthomonas virulence factor XopD.

PubMed: 17204475
DOI: 10.1074/jbc.M608730200

実験手法

X-RAY DIFFRACTION (1.8 Å)