2OFQ

NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system

2OFQ の概要

• エントリーDOI: 10.2210/pdb2ofq/pdb
• 分子名称: TraO, TraN (2 entities in total)
• 機能のキーワード: trao, tran, pkm101, protein transport-protein transport complex, protein transport/protein transport
• 由来する生物種: IncN plasmid R46; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13382.97

構造登録者

Harris, R.,Bayliss, R.,Driscoll, P.C.,Waksman, G. (登録日: 2007-01-04, 公開日: 2007-01-23, 最終更新日: 2023-12-27)

主引用文献

Bayliss, R.,Harris, R.,Coutte, L.,Monier, A.,Fronzes, R.,Christie, P.J.,Driscoll, P.C.,Waksman, G.
NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system
Proc.Natl.Acad.Sci.Usa, 104:1673-1678, 2007

PubMed Abstract: Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from 11 VirB subunits and VirD4. Two subunits, VirB9 and VirB7, form an important stabilizing complex in the outer membrane. We describe here the NMR structure of a complex between the C-terminal domain of the VirB9 homolog TraO (TraO(CT)), bound to VirB7-like TraN from plasmid pKM101. TraO(CT) forms a beta-sandwich around which TraN winds. Structure-based mutations in VirB7 and VirB9 of A. tumefaciens show that the heterodimer interface is conserved. Opposite this interface, the TraO structure shows a protruding three-stranded beta-appendage, and here, we supply evidence that the corresponding region of VirB9 of A. tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system.

PubMed: 17244707
DOI: 10.1073/pnas.0609535104

実験手法

SOLUTION NMR