2ODX

Solution structure of Zn(II)Cox4

2ODX の概要

• エントリーDOI: 10.2210/pdb2odx/pdb
• 分子名称: Cytochrome c oxidase polypeptide IV, ZINC ION (2 entities in total)
• 機能のキーワード: all beta-protein, metallo-protein, oxidoreductase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Mitochondrion inner membrane: P04037
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9062.58

構造登録者

Coyne III, J.H.,Ciofi-Baffoni, S. (登録日: 2006-12-27, 公開日: 2007-01-09, 最終更新日: 2023-12-27)

主引用文献

Coyne, H.J.,Ciofi-Baffoni, S.,Banci, L.,Bertini, I.,Zhang, L.,George, G.N.,Winge, D.R.
The characterization and role of zinc binding in yeast cox4.
J.Biol.Chem., 282:8926-8934, 2007

PubMed Abstract: Yeast Cox4 is a zinc binding subunit of cytochrome c oxidase. Cox4 is the only cofactor-containing subunit that is not directly part of the catalytic core of the enzyme located in the mitochondrial inner membrane. The Zn(II) site is shown to be distinct from the bovine ortholog, as it results from the x-ray structure of the entire cytochrome c oxidase in having a single histidyl residue and three conserved cysteines residues in the coordination sphere. Substitutions at the Cys ligand positions result in non-functional Cox4 proteins that fail to lead to cytochrome oxidase assembly. Limited function exists in His-119 mutants when overexpressed. Zn(II) binding in Cox4 is, therefore, important for the stability of the complex. The solution structure of yeast Cox4 elucidated by multidimensional NMR reveals a C-terminal globular domain consisting of two beta sheets analogous to the bovine ortholog except the loop containing the coordinating His in the yeast protein and the fourth Cys in the bovine protein are in different positions in the two structures. The conformation of this loop is dictated by the different sequence position of the fourth coordinating zinc ligand. The Zn(II) ion is buried within the domain, consistent with its role in structural stability. Potential functions of this matrix-facing subunit are discussed.

PubMed: 17215247
DOI: 10.1074/jbc.M610303200

実験手法

SOLUTION NMR