2ODH

Restriction Endonuclease BCNI in the Absence of DNA

2ODH の概要

• エントリーDOI: 10.2210/pdb2odh/pdb
• 関連するPDBエントリー: 2ODI
• 分子名称: R.BcnI, ACETATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: monomeric endonuclease, restriction enzyme, bcni, hydrolase
• 由来する生物種: Brevibacillus centrosporus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27499.74

構造登録者

Sokolowska, M.,Kaus-Drobek, M.,Czapinska, H.,Tamulaitis, G.,Szczepanowski, R.H.,Urbanke, K.,Siksnys, V.,Bochtler, M. (登録日: 2006-12-22, 公開日: 2007-03-20, 最終更新日: 2023-12-27)

主引用文献

Sokolowska, M.,Kaus-Drobek, M.,Czapinska, H.,Tamulaitis, G.,Szczepanowski, R.H.,Urbanke, C.,Siksnys, V.,Bochtler, M.
Monomeric restriction endonuclease BcnI in the apo form and in an asymmetric complex with target DNA.
J.Mol.Biol., 369:722-734, 2007

PubMed Abstract: Restriction endonuclease BcnI cleaves duplex DNA containing the sequence CC/SGG (S stands for C or G, / designates a cleavage position) to generate staggered products with single nucleotide 5'-overhangs. Here, we show that BcnI functions as a monomer that interacts with its target DNA in 1:1 molar ratio and report crystal structures of BcnI in the absence and in the presence of DNA. In the complex with DNA, BcnI makes specific contacts with all five bases of the target sequence and not just with a half-site, as the protomer of a typical dimeric restriction endonuclease. Our data are inconsistent with BcnI dimerization and suggest that the enzyme introduces double-strand breaks by sequentially nicking individual DNA strands, although this remains to be confirmed by kinetic experiments. BcnI is remotely similar to the DNA repair protein MutH and shares approximately 20% sequence identity with the restriction endonuclease MvaI, which is specific for the related sequence CC/WGG (W stands for A or T). As expected, BcnI is structurally similar to MvaI and recognizes conserved bases in the target sequence similarly but not identically. BcnI has a unique machinery for the recognition of the central base-pair.

PubMed: 17445830
DOI: 10.1016/j.jmb.2007.03.018

実験手法

X-RAY DIFFRACTION (1.6 Å)