2ODC

LEM-domain of the nuclear envelope protein emerin

2ODC の概要

• エントリーDOI: 10.2210/pdb2odc/pdb
• 関連するPDBエントリー: 2ODG
• 分子名称: Emerin (1 entity in total)
• 機能のキーワード: inner nuclear membrane protein, lem-domain multidimensional nmr dipolar couplings, membrane protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side: P50402
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5720.40

構造登録者

Clore, G.M.,Cai, M. (登録日: 2006-12-22, 公開日: 2007-03-13, 最終更新日: 2023-12-27)

主引用文献

Cai, M.,Huang, Y.,Suh, J.Y.,Louis, J.M.,Ghirlando, R.,Craigie, R.,Clore, G.M.
Solution NMR Structure of the Barrier-to-Autointegration Factor-Emerin Complex.
J.Biol.Chem., 282:14525-14535, 2007

PubMed Abstract: The barrier-to-autointegration factor BAF binds to the LEM domain (Em(LEM)) of the nuclear envelope protein emerin and plays an essential role in the nuclear architecture of metazoan cells. In addition, the BAF(2) dimer bridges and compacts double-stranded DNA nonspecifically via two symmetry-related DNA binding sites. In this article we present biophysical and structural studies on a complex of BAF(2) and Em(LEM). Light scattering, analytical ultracentrifugation, and NMR indicate a stoichiometry of one molecule of Em(LEM) bound per BAF(2) dimer. The equilibrium dissociation constant (K(d)) for the interaction of the BAF(2) dimer and Em(LEM), determined by isothermal titration calorimetry, is 0.59 +/- 0.03 microm. Z-exchange spectroscopy between corresponding cross-peaks of the magnetically non-equivalent subunits of the BAF(2) dimer in the complex yields a dissociation rate constant of 78 +/- 2s(-1). The solution NMR structure of the BAF(2)-Em(LEM) complex reveals that the LEM and DNA binding sites on BAF(2) are non-overlapping and that both subunits of the BAF(2) dimer contribute approximately equally to the Em(LEM) binding site. The relevance of the implications of the structural and biophysical data on the complex in the context of the interaction between the BAF(2) dimer and Em(LEM) at the nuclear envelope is discussed.

PubMed: 17355960
DOI: 10.1074/jbc.M700576200

実験手法

SOLUTION NMR