2OCJ

Human p53 core domain in the absence of DNA

2OCJ の概要

• エントリーDOI: 10.2210/pdb2ocj/pdb
• 分子名称: P53 TUMOR SUPPRESSOR, ZINC ION (3 entities in total)
• 機能のキーワード: tumor suppressor, p53, cancer, tetramer, dna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98609.14

構造登録者

Wang, Y.,Rosengarth, A.,Luecke, H. (登録日: 2006-12-20, 公開日: 2007-03-06, 最終更新日: 2023-08-30)

主引用文献

Wang, Y.,Rosengarth, A.,Luecke, H.
Structure of the human p53 core domain in the absence of DNA.
Acta Crystallogr.,Sect.D, 63:276-281, 2007

PubMed Abstract: The tumor suppressor protein p53 plays a key role in cell-cycle regulation by triggering DNA repair, cell-cycle arrest and apoptosis when the appropriate signal is received. p53 has the classic architecture of a transcription factor, with an amino-terminal transactivation domain, a core DNA-binding domain and carboxy-terminal tetramerization and regulatory domains. The crystal structure of the p53 core domain, which includes the amino acids from residue 96 to residue 289, has been determined in the absence of DNA to a resolution of 2.05 A. Crystals grew in a new monoclinic space group (P2(1)), with unit-cell parameters a = 68.91, b = 69.36, c = 84.18 A, beta = 90.11 degrees . The structure was solved by molecular replacement and has been refined to a final R factor of 20.9% (R(free) = 24.6%). The final model contains four molecules in the asymmetric unit with four zinc ions and 389 water molecules. The non-crystallographic tetramers display different protein contacts from those in other p53 crystals, giving rise to the question of how p53 arranges as a tetramer when it binds its target DNA.

PubMed: 17327663
DOI: 10.1107/S0907444906048499

実験手法

X-RAY DIFFRACTION (2.05 Å)