2OBU

Solution structure of GIP in TFE/water

2OBU の概要

• エントリーDOI: 10.2210/pdb2obu/pdb
• NMR情報: BMRB: 6681
• 分子名称: Gastric inhibitory polypeptide (1 entity in total)
• 機能のキーワード: gip; nmr; molecular modelling; helix; diabetes; obesity, hormone-growth factor complex, hormone/growth factor
• 細胞内の位置: Secreted: P09681
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4990.59

構造登録者

Alana, I.,Malthouse, J.P.G.,O'Harte, F.P.M.,Hewage, C.M. (登録日: 2006-12-20, 公開日: 2007-06-05, 最終更新日: 2023-12-27)

主引用文献

Alana, I.,Malthouse, J.P.G.,O'harte, F.P.M.,Hewage, C.M.
The bioactive conformation of glucose-dependent insulinotropic polypeptide by NMR and CD spectroscopy
Proteins, 68:92-99, 2007

PubMed Abstract: Glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal incretin hormone, which modulates physiological insulin secretion. Because of its glucose-sensitive insulinotropic activity, there has been a considerable interest in utilizing the hormone as a potential treatment for type 2 diabetes. Structural parameters obtained from NMR spectroscopy combined with molecular modeling techniques play a vital role in the design of new therapeutic drugs. Therefore, to understand the structural requirements for the biological activity of GIP, the solution structure of GIP was investigated by circular dichroism (CD) followed by proton nuclear magnetic resonance (NMR) spectroscopy. CD studies showed an increase in the helical character of the peptide with increasing concentration of trifluoroethanol (TFE) up to 50%. Therefore, the solution structure of GIP in 50% TFE was determined. It was found that there was an alpha-helix between residues 6 and 29, which tends to extend further up to residue 36. The implications of the C-terminal extended helical segment in the inhibitory properties of GIP on gastric acid secretion are discussed. It is shown that the adoption by GIP of an alpha-helical secondary structure is a requirement for its biological activity. Knowledge of the solution structure of GIP will help in the understanding of how the peptide interacts with its receptor and aids in the design of new therapeutic agents useful for the treatment of diabetes.

PubMed: 17393464
DOI: 10.1002/prot.21372

実験手法

SOLUTION NMR