2OBD

Crystal Structure of Cholesteryl Ester Transfer Protein

2OBD の概要

• エントリーDOI: 10.2210/pdb2obd/pdb
• 分子名称: Cholesteryl ester transfer protein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (9 entities in total)
• 機能のキーワード: cholesteryl ester, lipid transfer protein, lipid transport
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57489.27

構造登録者

Qiu, X. (登録日: 2006-12-18, 公開日: 2007-01-23, 最終更新日: 2024-11-13)

主引用文献

Qiu, X.,Mistry, A.,Ammirati, M.J.,Chrunyk, B.A.,Clark, R.W.,Cong, Y.,Culp, J.S.,Danley, D.E.,Freeman, T.B.,Geoghegan, K.F.,Griffor, M.C.,Hawrylik, S.J.,Hayward, C.M.,Hensley, P.,Hoth, L.R.,Karam, G.A.,Lira, M.E.,Lloyd, D.B.,McGrath, K.M.,Stutzman-Engwall, K.J.,Subashi, A.K.,Subashi, T.A.,Thompson, J.F.,Wang, I.K.,Zhao, H.,Seddon, A.P.
Crystal structure of cholesteryl ester transfer protein reveals a long tunnel and four bound lipid molecules.
Nat.Struct.Mol.Biol., 14:106-113, 2007

PubMed Abstract: Cholesteryl ester transfer protein (CETP) shuttles various lipids between lipoproteins, resulting in the net transfer of cholesteryl esters from atheroprotective, high-density lipoproteins (HDL) to atherogenic, lower-density species. Inhibition of CETP raises HDL cholesterol and may potentially be used to treat cardiovascular disease. Here we describe the structure of CETP at 2.2-A resolution, revealing a 60-A-long tunnel filled with two hydrophobic cholesteryl esters and plugged by an amphiphilic phosphatidylcholine at each end. The two tunnel openings are large enough to allow lipid access, which is aided by a flexible helix and possibly also by a mobile flap. The curvature of the concave surface of CETP matches the radius of curvature of HDL particles, and potential conformational changes may occur to accommodate larger lipoprotein particles. Point mutations blocking the middle of the tunnel abolish lipid-transfer activities, suggesting that neutral lipids pass through this continuous tunnel.

PubMed: 17237796
DOI: 10.1038/nsmb1197

実験手法

X-RAY DIFFRACTION (2.1 Å)