2OB7

Structure of tmRNA-(SmpB)2 complex as inferred from cryo-EM

2OB7 の概要

• エントリーDOI: 10.2210/pdb2ob7/pdb
• 関連するPDBエントリー: 1N34 1PV6 1Q2B
• EMDBエントリー: 1310 1311 1312
• 分子名称: transfer-messenger RNA, 16S ribosomal RNA, SsrA-binding protein (3 entities in total)
• 機能のキーワード: tmrna, smpb, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 170597.87

構造登録者

Frank, J.,Felden, B.,Gillet, R.,Li, W. (登録日: 2006-12-18, 公開日: 2007-01-23, 最終更新日: 2023-12-27)

主引用文献

Gillet, R.,Kaur, S.,Li, W.,Hallier, M.,Felden, B.,Frank, J.
Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.
J.Biol.Chem., 282:6356-6363, 2007

PubMed Abstract: A eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer-messenger RNA, small protein B, and ribosome protein S1. By means of cryo-electron microscopy, we obtained a map of the complex composed of a stalled ribosome and small protein B, which appears near the decoding center. This result suggests that, when lacking a codon, the A-site on the small subunit is a target for small protein B. To investigate the role of S1 played in trans-translation, we obtained a cryo-electron microscopic map, including a stalled ribosome, transfer-messenger RNA, and small protein Bs but in the absence of S1. In this complex, several connections between the 30 S subunit and transfer-messenger RNA that appear in the +S1 complex are no longer found. We propose the unifying concept of scaffolding for the roles of small protein B and S1 in binding of transfer-messenger RNA to the ribosome during trans-translation, and we infer a pathway of sequential binding events in the initial phase of trans-translation.

PubMed: 17179154
DOI: 10.1074/jbc.M609658200

実験手法

ELECTRON MICROSCOPY (13.6 Å)