2O8K

NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element

2O8K の概要

• エントリーDOI: 10.2210/pdb2o8k/pdb
• NMR情報: BMRB: 15083
• 分子名称: 5'-D(*TP*TP*TP*TP*GP*GP*CP*AP*CP*GP*TP*TP*TP*C)-3', 5'-D(*GP*AP*AP*AP*CP*GP*TP*GP*CP*CP*AP*AP*AP*A)-3', RNA polymerase sigma factor RpoN (3 entities in total)
• 機能のキーワード: protein-dna complex, helix-turn-helix, transcription factor, sigma-54, rna polymerase, transcription-dna complex, transcription/dna
• 由来する生物種: Aquifex aeolicus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 16048.41

構造登録者

Doucleff, M.,Pelton, J.G.,Lee, P.S.,Wemmer, D.E. (登録日: 2006-12-12, 公開日: 2007-07-17, 最終更新日: 2023-12-27)

主引用文献

Doucleff, M.,Pelton, J.G.,Lee, P.S.,Nixon, B.T.,Wemmer, D.E.
Structural basis of DNA recognition by the alternative sigma-factor, sigma54.
J.Mol.Biol., 369:1070-1078, 2007

PubMed Abstract: The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.

PubMed: 17481658
DOI: 10.1016/j.jmb.2007.04.019

実験手法

SOLUTION NMR