2O4F

Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions

Summary for 2O4F

• Entry DOI: 10.2210/pdb2o4f/pdb
• Descriptor: 5'-D(*TP*GP*GP*GP*GP*T)-3', SODIUM ION, LITHIUM ION, ... (4 entities in total)
• Functional Keywords: dna, quadruple helix, parallel-stranded
• Total number of polymer chains: 8
• Total formula weight: 15209.88

Authors

Creze, C.,Rinaldi, B.,Haser, R.,Bouvet, P.,Gouet, P. (deposition date: 2006-12-04, release date: 2007-05-22, Last modification date: 2023-08-30)

Primary citation

Creze, C.,Rinaldi, B.,Haser, R.,Bouvet, P.,Gouet, P.
Structure of a d(TGGGGT) quadruplex crystallized in the presence of Li+ ions.
Acta Crystallogr.,Sect.D, 63:682-688, 2007

PubMed Abstract: A parallel 5'-d(TGGGGT)-3' quadruplex was formed in Na(+) solution and crystallized using lithium sulfate as the main precipitating agent. The X-ray structure was determined to 1.5 A resolution in space group P2(1) by molecular replacement. The asymmetric unit consists of a characteristic motif of two quadruplexes stacked at their 5' ends. All nucleotides are clearly defined in the density and could be positioned. A single bound Li(+) ion is observed at the surface of the column formed by the two joined molecules. Thus, this small alkali metal ion appears to be unsuitable as a replacement for the Na(+) ion in the central channel of G-quartets, unlike K(+) or Tl(+) ions. A well conserved constellation of water molecules is observed in the grooves of the dimeric structure.

PubMed: 17505106
DOI: 10.1107/S0907444907013315

Experimental method

X-RAY DIFFRACTION (1.5 Å)