2O4F

Structure of a parallel-stranded guanine tetraplex crystallised with monovalent ions

2O4F の概要

• エントリーDOI: 10.2210/pdb2o4f/pdb
• 分子名称: 5'-D(*TP*GP*GP*GP*GP*T)-3', SODIUM ION, LITHIUM ION, ... (4 entities in total)
• 機能のキーワード: dna, quadruple helix, parallel-stranded
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 15209.88

構造登録者

Creze, C.,Rinaldi, B.,Haser, R.,Bouvet, P.,Gouet, P. (登録日: 2006-12-04, 公開日: 2007-05-22, 最終更新日: 2023-08-30)

主引用文献

Creze, C.,Rinaldi, B.,Haser, R.,Bouvet, P.,Gouet, P.
Structure of a d(TGGGGT) quadruplex crystallized in the presence of Li+ ions.
Acta Crystallogr.,Sect.D, 63:682-688, 2007

PubMed Abstract: A parallel 5'-d(TGGGGT)-3' quadruplex was formed in Na(+) solution and crystallized using lithium sulfate as the main precipitating agent. The X-ray structure was determined to 1.5 A resolution in space group P2(1) by molecular replacement. The asymmetric unit consists of a characteristic motif of two quadruplexes stacked at their 5' ends. All nucleotides are clearly defined in the density and could be positioned. A single bound Li(+) ion is observed at the surface of the column formed by the two joined molecules. Thus, this small alkali metal ion appears to be unsuitable as a replacement for the Na(+) ion in the central channel of G-quartets, unlike K(+) or Tl(+) ions. A well conserved constellation of water molecules is observed in the grooves of the dimeric structure.

PubMed: 17505106
DOI: 10.1107/S0907444907013315

実験手法

X-RAY DIFFRACTION (1.5 Å)