2O3X

Crystal Structure of the Prokaryotic Ribosomal Decoding Site Complexed with Paromamine Derivative NB30

2O3X の概要

• エントリーDOI: 10.2210/pdb2o3x/pdb
• 関連するPDBエントリー: 2O3V 2O3W 2O3Y
• 分子名称: RNA (5'-R(*UP*UP*GP*CP*GP*UP*CP*AP*CP*AP*CP*CP*GP*GP*UP*GP*AP*AP*GP*UP*CP*GP*C)-3'), (1R,2R,3S,4R,6S)-4,6-DIAMINO-2-[(5-AMINO-5-DEOXY-BETA-D-RIBOFURANOSYL)OXY]-3-HYDROXYCYCLOHEXYL 2-AMINO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE (2 entities in total)
• 機能のキーワード: aminoglycoside, antibiotics, ribosome, decoding site, prokaryote, translation inhibition, stop codon readthrough, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15165.29

構造登録者

Kondo, J.,Hainrichson, M.,Nudelman, I.,Shallom-Shezifi, D.,Baasov, T.,Westhof, E. (登録日: 2006-12-02, 公開日: 2007-11-06, 最終更新日: 2023-08-30)

主引用文献

Kondo, J.,Hainrichson, M.,Nudelman, I.,Shallom-Shezifi, D.,Barbieri, C.M.,Pilch, D.S.,Westhof, E.,Baasov, T.
Differential Selectivity of Natural and Synthetic Aminoglycosides towards the Eukaryotic and Prokaryotic Decoding A Sites.
Chembiochem, 8:1700-1709, 2007

PubMed Abstract: The lack of absolute prokaryotic selectivity of natural antibiotics is widespread and is a significant clinical problem. The use of this disadvantage of aminoglycoside antibiotics for the possible treatment of human genetic diseases is extremely challenging. Here, we have used a combination of biochemical and structural analysis to compare and contrast the molecular mechanisms of action and the structure-activity relationships of a new synthetic aminoglycoside, NB33, and a structurally similar natural aminoglycoside apramycin. The data presented herein demonstrate the general molecular principles that determine the decreased selectivity of apramycin for the prokaryotic decoding site, and the increased selectivity of NB33 for the eukaryotic decoding site. These results are therefore extremely beneficial for further research on both the design of new aminoglycoside-based antibiotics with diminished deleterious effects on humans, as well as the design of new aminoglycoside-based structures that selectively target the eukaryotic ribosome.

PubMed: 17705310
DOI: 10.1002/cbic.200700271

実験手法

X-RAY DIFFRACTION (2.9 Å)