2O3D

Structure of human SF2/ASF RNA recognition motif 2 (RRM2)

2O3D の概要

• エントリーDOI: 10.2210/pdb2o3d/pdb
• NMR情報: BMRB: 7301
• 分子名称: Splicing factor, arginine/serine-rich 1 (1 entity in total)
• 機能のキーワード: rrm domain, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q07955
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12930.45

構造登録者

Tintaru, A.M.,Hautbergue, G.M.,Hounslow, A.M.,Lian, L.Y.,Craven, C.J.,Wilson, S.A. (登録日: 2006-12-01, 公開日: 2007-10-16, 最終更新日: 2023-12-27)

主引用文献

Tintaru, A.M.,Hautbergue, G.M.,Hounslow, A.M.,Hung, M.L.,Lian, L.Y.,Craven, C.J.,Wilson, S.A.
Structural and functional analysis of RNA and TAP binding to SF2/ASF.
Embo Rep., 8:756-762, 2007

PubMed Abstract: The serine/arginine-rich (SR) protein splicing factor 2/alternative splicing factor (SF2/ASF) has a role in splicing, stability, export and translation of messenger RNA. Here, we present the structure of the RNA recognition motif (RRM) 2 from SF2/ASF, which has an RRM fold with a considerably extended loop 5 region, containing a two-stranded beta-sheet. The loop 5 extension places the previously identified SR protein kinase 1 docking sequence largely within the RRM fold. We show that RRM2 binds to RNA in a new way, by using a tryptophan within a conserved SWQLKD motif that resides on helix alpha1, together with amino acids from strand beta2 and a histidine on loop 5. The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2.

PubMed: 17668007
DOI: 10.1038/sj.embor.7401031

実験手法

SOLUTION NMR