2O1I

RH(BPY)2CHRYSI complexed to mismatched DNA

2O1I の概要

• エントリーDOI: 10.2210/pdb2o1i/pdb
• 分子名称: 5'-D(*CP*GP*GP*AP*AP*AP*TP*TP*CP*CP*CP*G)-3', bis(2,2'-bipyridine-kappa~2~N~1~,N~1'~)[chrysene-5,6-diiminato(2-)-kappa~2~N,N']rhodium(4+) (3 entities in total)
• 機能のキーワード: dna mismatch, metallointercalator, dna recognition, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5656.07

構造登録者

Pierre, V.C.,Kaiser, J.T.,Barton, J.K. (登録日: 2006-11-28, 公開日: 2007-01-09, 最終更新日: 2023-12-27)

主引用文献

Pierre, V.C.,Kaiser, J.T.,Barton, J.K.
Insights into finding a mismatch through the structure of a mispaired DNA bound by a rhodium intercalator.
Proc.Natl.Acad.Sci.Usa, 104:429-434, 2007

PubMed Abstract: We report the 1.1-A resolution crystal structure of a bulky rhodium complex bound to two different DNA sites, mismatched and matched in the oligonucleotide 5'-(dCGGAAATTCCCG)2-3'. At the AC mismatch site, the structure reveals ligand insertion from the minor groove with ejection of both mismatched bases and elucidates how destabilized mispairs in DNA may be recognized. This unique binding mode contrasts with major groove intercalation, observed at a matched site, where doubling of the base pair rise accommodates stacking of the intercalator. Mass spectral analysis reveals different photocleavage products associated with the two binding modes in the crystal, with only products characteristic of mismatch binding in solution. This structure, illustrating two clearly distinct binding modes for a molecule with DNA, provides a rationale for the interrogation and detection of mismatches.

PubMed: 17194756
DOI: 10.1073/pnas.0610170104

実験手法

X-RAY DIFFRACTION (1.1 Å)