2O0F

Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3

2O0F の概要

• エントリーDOI: 10.2210/pdb2o0f/pdb
• 関連するPDBエントリー: 2H5E
• EMDBエントリー: 1302
• 分子名称: Peptide chain release factor 3 (1 entity in total)
• 機能のキーワード: rf3, ribosome, cryo-em, translation
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 59651.93

構造登録者

Gao, H.,Zhou, Z.,Rawat, U.,Huang, C.,Bouakaz, L.,Wang, C.,Liu, Y.,Zavialov, A.,Gursky, R.,Sanyal, S.,Ehrenberg, M.,Frank, J.,Song, H. (登録日: 2006-11-27, 公開日: 2007-07-24, 最終更新日: 2023-12-27)

主引用文献

Gao, H.,Zhou, Z.,Rawat, U.,Huang, C.,Bouakaz, L.,Wang, C.,Cheng, Z.,Liu, Y.,Zavialov, A.,Gursky, R.,Sanyal, S.,Ehrenberg, M.,Frank, J.,Song, H.
RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors
Cell(Cambridge,Mass.), 129:929-941, 2007

PubMed Abstract: During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.

PubMed: 17540173
DOI: 10.1016/j.cell.2007.03.050

実験手法

ELECTRON MICROSCOPY (15.5 Å)