2O07

Human spermidine synthase

2O07 の概要

• エントリーDOI: 10.2210/pdb2o07/pdb
• 関連するPDBエントリー: 2O05 2O06 2O0L
• 分子名称: Spermidine synthase, SPERMIDINE, 5'-DEOXY-5'-METHYLTHIOADENOSINE, ... (4 entities in total)
• 機能のキーワード: spermidine synthase, structural genomics, structural genomics consortium, sgc, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68888.65

構造登録者

Min, J.,Wu, H.,Zeng, H.,Loppnau, P.,Weigelt, J.,Sundstrom, M.,Arrowsmith, C.H.,Edwards, A.M.,Bochkarev, A.,Plotnikov, A.N.,Structural Genomics Consortium (SGC) (登録日: 2006-11-27, 公開日: 2006-12-12, 最終更新日: 2023-12-27)

主引用文献

Wu, H.,Min, J.,Ikeguchi, Y.,Zeng, H.,Dong, A.,Loppnau, P.,Pegg, A.E.,Plotnikov, A.N.
Structure and mechanism of spermidine synthases.
Biochemistry, 46:8331-8339, 2007

PubMed Abstract: Aminopropyltransferases transfer aminopropyl groups from decarboxylated S-adenosylmethionine to amine acceptors, forming polyamines. Structural and biochemical studies have been carried out with the human spermidine synthase, which is highly specific for putrescine as the amine acceptor, and the Thermotoga maritima spermidine synthase, which prefers putrescine but is more tolerant of other substrates. Comparison of the structures of the human spermidine synthase with both substrates and products with the known structure of T. maritima spermidine synthase complexed to a multisubstrate analogue inhibitor and analysis of the properties of site-directed mutants provide a general mechanistic hypothesis for the aminopropyl transfer reaction. The studies also provide a structural basis for the specificity of the spermidine synthase subclass of the aminopropyltransferase family.

PubMed: 17585781
DOI: 10.1021/bi602498k

実験手法

X-RAY DIFFRACTION (1.89 Å)