2NR0

Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA

2NR0 の概要

• エントリーDOI: 10.2210/pdb2nr0/pdb
• 関連するPDBエントリー: 1DJ0 2NQP 2NRE
• 分子名称: leucyl tRNA, tRNA pseudouridine synthase A (2 entities in total)
• 機能のキーワード: pseudouridine synthase, anticodon stem loop, trna, multisite specificity, isomerase-rna complex, isomerase/rna
• 由来する生物種: Escherichia coli K12; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 234145.04

構造登録者

Hur, S.,Stroud, R.M. (登録日: 2006-11-01, 公開日: 2007-05-15, 最終更新日: 2023-08-30)

主引用文献

Hur, S.,Stroud, R.M.
How U38, 39, and 40 of Many tRNAs Become the Targets for Pseudouridylation by TruA.
Mol.Cell, 26:189-203, 2007

PubMed Abstract: Translational accuracy and efficiency depend upon modification of uridines in the tRNA anticodon stem loop (ASL) by a highly conserved pseudouridine synthase TruA. TruA specifically modifies uridines at positions 38, 39, and/or 40 of tRNAs with highly divergent sequences and structures through a poorly characterized mechanism that differs from previously studied RNA-modifying enzymes. The molecular basis for the site and substrate "promiscuity" was studied by determining the crystal structures of E. coli TruA in complex with two different leucyl tRNAs in conjunction with functional assays and computer simulation. The structures capture three stages of the TruA*tRNA reaction, revealing the mechanism by which TruA selects the target site. We propose that TruA utilizes the intrinsic flexibility of the ASL for site promiscuity and also to select against intrinsically stable tRNAs to avoid their overstabilization through pseudouridylation, thereby maintaining the balance between the flexibility and stability required for its biological function.

PubMed: 17466622
DOI: 10.1016/j.molcel.2007.02.027

実験手法

X-RAY DIFFRACTION (3.9 Å)