2NPR

Structural Studies on Plasmodium vivax Merozoite Surface Protein-1

2NPR の概要

• エントリーDOI: 10.2210/pdb2npr/pdb
• NMR情報: BMRB: 15007
• 分子名称: Merozoite surface protein 1 (1 entity in total)
• 機能のキーワード: egf-like domain, membrane protein
• 由来する生物種: Plasmodium vivax (strain Belem)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9823.01

構造登録者

Babon, J.J.,Morgan, W.D.,Kelly, G.,Eccleston, J.F.,Feeney, J.,Holder, A.A. (登録日: 2006-10-29, 公開日: 2007-03-20, 最終更新日: 2024-11-13)

主引用文献

Babon, J.J.,Morgan, W.D.,Kelly, G.,Eccleston, J.F.,Feeney, J.,Holder, A.A.
Structural studies on Plasmodium vivax merozoite surface protein-1
Mol.Biochem.Parasitol., 153:31-40, 2007

PubMed Abstract: Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the complex to be shed and leaving only a small 15kDa sub-unit, MSP-1(19), on the merozite surface. MSP-1(19) is considered a strong vaccine candidate. We have determined the solution structure of MSP-1(19) from P. vivax using nuclear magnetic resonance (NMR) and show that, like in other Plasmodium species, it consists of two EGF-like domains that are oriented head-to-tail. The protein has a flat, disk-like shape with a highly charged surface. When MSP-1(19) is part of the larger MSP-1(42) precursor it exists as an independent domain with no stable contacts to the rest of the sub-unit. Gel filtration and analytical ultracentrifugation experiments indicate that P. vivax MSP-1(42) exists as a dimer in solution. MSP-1(19) itself is a monomer, however, 35 amino-acids immediately upstream of its N-terminus are sufficient to cause dimerization. Our data suggest that if MSP-1(42) exists as a dimer in vivo, secondary processing would cause the dissociation of two tightly linked MSP-1(19) proteins on the merozoite surface just prior to invasion.

PubMed: 17343930
DOI: 10.1016/j.molbiopara.2007.01.015

実験手法

SOLUTION NMR