2NP2

Hbb-DNA complex

2NP2 の概要

• エントリーDOI: 10.2210/pdb2np2/pdb
• 関連するPDBエントリー: 1B8Z 1IHF 1OWF 1P51 1P71 1P78
• 分子名称: 36-MER, Hbb (3 entities in total)
• 機能のキーワード: protein-dna complex, dna-binding protein, dna-bending protein, dnabii family, hu/ihf family, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Borrelia burgdorferi (Lyme disease spirochete)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47513.85

構造登録者

Rice, P.A.,Mouw, K.W. (登録日: 2006-10-26, 公開日: 2007-02-27, 最終更新日: 2023-08-30)

主引用文献

Mouw, K.W.,Rice, P.A.
Shaping the Borrelia burgdorferi genome: crystal structure and binding properties of the DNA-bending protein Hbb.
Mol.Microbiol., 63:1319-1330, 2007

PubMed Abstract: The genome of the Lyme disease-causing spirochete Borrelia burgdorferi encodes only a single polypeptide from the integration host factor (IHF)/HU or 'DNABII' family of nucleoid-associated proteins - Hbb. DNABII proteins induce large bends in DNA and serve as architectural factors in a variety of prokaryotic cellular processes. We have solved the crystal structure of an Hbb-DNA complex in which the DNA is bent by over 180 degrees . We find that like IHF, Hbb relies exclusively on indirect readout to recognize its cognate site. Additional binding studies show that the sequence preferences of Hbb are related to, yet distinct from those of IHF. Defining these binding characteristics may help to uncover additional roles for Hbb in Borrelia DNA metabolism as well as further our understanding of the mechanism of indirect readout.

PubMed: 17244195
DOI: 10.1111/j.1365-2958.2007.05586.x

実験手法

X-RAY DIFFRACTION (3.02 Å)