2NLR

STREPTOMYCES LIVIDANS ENDOGLUCANASE (EC: 3.2.1.4) COMPLEX WITH MODIFIED GLUCOSE TRIMER

2NLR の概要

• エントリーDOI: 10.2210/pdb2nlr/pdb
• 分子名称: PROTEIN (ENDOGLUCANASE (E.C.3.2.1.4)), beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-2-deoxy-2-fluoro-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: hydrolase (endoglucanase), glycosyl hydrolase, family 12, endoglucanase, celb2, glycosyl-enzyme intermediate, hydrolase
• 由来する生物種: Streptomyces lividans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25085.14

構造登録者

Sulzenbacher, G.,Dupont, C.,Davies, G.J. (登録日: 1998-11-02, 公開日: 1999-11-10, 最終更新日: 2024-10-30)

主引用文献

Sulzenbacher, G.,Mackenzie, L.F.,Wilson, K.S.,Withers, S.G.,Dupont, C.,Davies, G.J.
The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution.
Biochemistry, 38:4826-4833, 1999

PubMed Abstract: Glycoside hydrolases have been classified into over 66 families on the basis of amino acid sequence. Recently a number of these families have been grouped into "clans" which share a common fold and catalytic mechanism [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. Glycoside hydrolase Clan GH-C groups family 11 xylanases and family 12 cellulases, which share the same jellyroll topology, with two predominantly antiparallel beta-sheets forming a long substrate-binding cleft, and act with net retention of anomeric configuration. Here we present the three-dimensional structure of a family 12 endoglucanase, Streptomyces lividans CelB2, in complex with a 2-deoxy-2-fluorocellotrioside. Atomic resolution (1.2 A) data allow clear identification of two distinct species in the crystal. One is the glycosyl-enzyme intermediate, with the mechanism-based inhibitor covalently linked to the nucleophile Glu 120, and the other a complex with the reaction product, 2-deoxy-2-fluoro-beta-D-cellotriose. The active site architecture of the complex provides insight into the double-displacement mechanism of retaining glycoside hydrolases and also sheds light on the basis of the differences in specificity between family 12 cellulases and family 11 xylanases.

PubMed: 10200171
DOI: 10.1021/bi982648i

実験手法

X-RAY DIFFRACTION (1.2 Å)