2NC7

Antimicrobial peptide protegrin PG-5

2NC7 の概要

• エントリーDOI: 10.2210/pdb2nc7/pdb
• NMR情報: BMRB: 26009
• 分子名称: Protegrin-5 (1 entity in total)
• 機能のキーワード: protegrin, antimicrobial peptide, dpc micelle, antimicrobial protein
• 由来する生物種: Sus scrofa (pigs,swine,wild boar)
• 細胞内の位置: Secreted: P49934
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2106.59

構造登録者

Kolosova, O.A.,Klochkova, E.A.,Aganov, A.V.,Klochkov, V.V. (登録日: 2016-03-21, 公開日: 2017-04-19, 最終更新日: 2024-11-27)

主引用文献

Usachev, K.S.,Kolosova, O.A.,Klochkova, E.A.,Yulmetov, A.R.,Aganov, A.V.,Klochkov, V.V.
Oligomerization of the antimicrobial peptide Protegrin-5 in a membrane-mimicking environment. Structural studies by high-resolution NMR spectroscopy.
Eur. Biophys. J., 46:293-300, 2017

PubMed Abstract: Protegrin pore formation is believed to occur in a stepwise fashion that begins with a nonspecific peptide interaction with the negatively charged bacterial cell walls via hydrophobic and positively charged amphipathic surfaces. There are five known nature protegrins (PG1-PG5), and early studies of PG-1 (PDB ID:1PG1) shown that it could form antiparallel dimer in membrane mimicking environment which could be a first step for further oligomeric membrane pore formation. Later, we solved PG-2 (PDB ID:2MUH) and PG-3 (PDB ID:2MZ6) structures in the same environment and for PG-3 observed a strong d NOE effects between residues R18 and F12, V14, and V16. These "inconsistent" with monomer structure NOEs appears due to formation of an additional antiparallel β-sheet between two monomers. It was also suggested that there is a possible association of protegrins dimers to form octameric or decameric β-barrels in an oligomer state. In order to investigate a more detailed oligomerization process of protegrins, in the present article we report the monomer (PDB ID: 2NC7) and octamer pore structures of the protegrin-5 (PG-5) in the presence of DPC micelles studied by solution NMR spectroscopy. In contrast to PG-1, PG-2, and PG-3 studies, for PG-5 we observed not only dimer NOEs but also several additional NOEs between side chains, which allows us to calculate an octamer pore structure of PG-5 that was in good agreement with previous AFM and PMF data.

PubMed: 27589857
DOI: 10.1007/s00249-016-1167-5

実験手法

SOLUTION NMR