2NBQ

NMR Structure of the C-Terminal Domain of human APOBEC3B

2NBQ の概要

• エントリーDOI: 10.2210/pdb2nbq/pdb
• NMR情報: BMRB: 25985
• 分子名称: DNA dC->dU-editing enzyme APOBEC-3B, ZINC ION (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus : Q9UH17
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24501.10

構造登録者

Byeon, I.L.,Byeon, C.,Gronenborn, A.M. (登録日: 2016-03-09, 公開日: 2016-06-01, 最終更新日: 2024-05-15)

主引用文献

Byeon, I.J.,Byeon, C.H.,Wu, T.,Mitra, M.,Singer, D.,Levin, J.G.,Gronenborn, A.M.
Nuclear Magnetic Resonance Structure of the APOBEC3B Catalytic Domain: Structural Basis for Substrate Binding and DNA Deaminase Activity.
Biochemistry, 55:2944-2959, 2016

PubMed Abstract: Human APOBEC3B (A3B) is a member of the APOBEC3 (A3) family of cytidine deaminases, which function as DNA mutators and restrict viral pathogens and endogenous retrotransposons. Recently, A3B was identified as a major source of genetic heterogeneity in several human cancers. Here, we determined the solution nuclear magnetic resonance structure of the catalytically active C-terminal domain (CTD) of A3B and performed detailed analyses of its deaminase activity. The core of the structure comprises a central five-stranded β-sheet with six surrounding helices, common to all A3 proteins. The structural fold is most similar to that of A3A and A3G-CTD, with the most prominent difference being found in loop 1. The catalytic activity of A3B-CTD is ∼15-fold lower than that of A3A, although both exhibit a similar pH dependence. Interestingly, A3B-CTD with an A3A loop 1 substitution had significantly increased deaminase activity, while a single-residue change (H29R) in A3A loop 1 reduced A3A activity to the level seen with A3B-CTD. This establishes that loop 1 plays an important role in A3-catalyzed deamination by precisely positioning the deamination-targeted C into the active site. Overall, our data provide important insights into the determinants of the activities of individual A3 proteins and facilitate understanding of their biological function.

PubMed: 27163633
DOI: 10.1021/acs.biochem.6b00382

実験手法

SOLUTION NMR