2NBG
Structure of the Geobacillus stearothermophilus IF2 G3-subdomain
Summary for 2NBG
| Entry DOI | 10.2210/pdb2nbg/pdb |
| NMR Information | BMRB: 25975 |
| Descriptor | Translation initiation factor IF-2 (1 entity in total) |
| Functional Keywords | ribosome, translation initiation, translation |
| Biological source | Geobacillus stearothermophilus |
| Cellular location | Cytoplasm: P04766 |
| Total number of polymer chains | 1 |
| Total formula weight | 13649.75 |
| Authors | Dongre, R.,Folkers, G.E.,Gualerzi, C.O.,Boelens, R.,Wienk, H. (deposition date: 2016-02-11, release date: 2016-07-20, Last modification date: 2024-05-15) |
| Primary citation | Dongre, R.,Folkers, G.E.,Gualerzi, C.O.,Boelens, R.,Wienk, H. A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit. Protein Sci., 25:1722-1733, 2016 Cited by PubMed Abstract: Bacterial translation initiation factor IF2 complexed with GTP binds to the 30S ribosomal subunit, promotes ribosomal binding of fMet-tRNA, and favors the joining of the small and large ribosomal subunits yielding a 70S initiation complex ready to enter the translation elongation phase. Within the IF2 molecule subdomain G3, which is believed to play an important role in the IF2-30S interaction, is positioned between the GTP-binding G2 and the fMet-tRNA binding C-terminal subdomains. In this study the solution structure of subdomain G3 of Geobacillus stearothermophilus IF2 has been elucidated. G3 forms a core structure consisting of two β-sheets with each four anti-parallel strands, followed by a C-terminal α-helix. In line with its role as linker between G3 and subdomain C1, this helix has no well-defined orientation but is endowed with a dynamic nature. The structure of the G3 core is that of a typical OB-fold module, similar to that of the corresponding subdomain of Thermus thermophilus IF2, and to that of other known RNA-binding modules such as IF2-C2, IF1 and subdomains II of elongation factors EF-Tu and EF-G. Structural comparisons have resulted in a model that describes the interaction between IF2-G3 and the 30S ribosomal subunit. PubMed: 27364543DOI: 10.1002/pro.2977 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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