2NBG

Structure of the Geobacillus stearothermophilus IF2 G3-subdomain

2NBG の概要

• エントリーDOI: 10.2210/pdb2nbg/pdb
• NMR情報: BMRB: 25975
• 分子名称: Translation initiation factor IF-2 (1 entity in total)
• 機能のキーワード: ribosome, translation initiation, translation
• 由来する生物種: Geobacillus stearothermophilus
• 細胞内の位置: Cytoplasm: P04766
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13649.75

構造登録者

Dongre, R.,Folkers, G.E.,Gualerzi, C.O.,Boelens, R.,Wienk, H. (登録日: 2016-02-11, 公開日: 2016-07-20, 最終更新日: 2024-05-15)

主引用文献

Dongre, R.,Folkers, G.E.,Gualerzi, C.O.,Boelens, R.,Wienk, H.
A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit.
Protein Sci., 25:1722-1733, 2016

PubMed Abstract: Bacterial translation initiation factor IF2 complexed with GTP binds to the 30S ribosomal subunit, promotes ribosomal binding of fMet-tRNA, and favors the joining of the small and large ribosomal subunits yielding a 70S initiation complex ready to enter the translation elongation phase. Within the IF2 molecule subdomain G3, which is believed to play an important role in the IF2-30S interaction, is positioned between the GTP-binding G2 and the fMet-tRNA binding C-terminal subdomains. In this study the solution structure of subdomain G3 of Geobacillus stearothermophilus IF2 has been elucidated. G3 forms a core structure consisting of two β-sheets with each four anti-parallel strands, followed by a C-terminal α-helix. In line with its role as linker between G3 and subdomain C1, this helix has no well-defined orientation but is endowed with a dynamic nature. The structure of the G3 core is that of a typical OB-fold module, similar to that of the corresponding subdomain of Thermus thermophilus IF2, and to that of other known RNA-binding modules such as IF2-C2, IF1 and subdomains II of elongation factors EF-Tu and EF-G. Structural comparisons have resulted in a model that describes the interaction between IF2-G3 and the 30S ribosomal subunit.

PubMed: 27364543
DOI: 10.1002/pro.2977

実験手法

SOLUTION NMR