2NB5

NMR solution structure of PawS Derived Peptide 9 (PDP-9)

2NB5 の概要

• エントリーDOI: 10.2210/pdb2nb5/pdb
• 関連するPDBエントリー: 2NB6
• NMR情報: BMRB: 25962
• 分子名称: Preproalbumin PawS1 (1 entity in total)
• 機能のキーワード: cyclic backbone, plant peptide, plant protein
• 由来する生物種: Perymenium macrocephalum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1938.10

構造登録者

Armstrong, D.A.,Franke, B.,Elliott, A.G.,Mylne, J.S.,Rosengren, K.J. (登録日: 2016-01-24, 公開日: 2016-06-29, 最終更新日: 2024-11-20)

主引用文献

Elliott, A.G.,Franke, B.,Armstrong, D.A.,Craik, D.J.,Mylne, J.S.,Rosengren, K.J.
Natural structural diversity within a conserved cyclic peptide scaffold.
Amino Acids, 49:103-116, 2017

PubMed Abstract: We recently isolated and described the evolutionary origin of a diverse class of small single-disulfide bonded peptides derived from Preproalbumin with SFTI-1 (PawS1) proteins in the seeds of flowering plants (Asteraceae). The founding member of the PawS derived peptide (PDP) family is the potent trypsin inhibitor SFTI-1 (sunflower trypsin inhibitor-1) from Helianthus annuus, the common sunflower. Here we provide additional structures and describe the structural diversity of this new class of small peptides, derived from solution NMR studies, in detail. We show that although most have a similar backbone framework with a single disulfide bond and in many cases a head-to-tail cyclized backbone, they all have their own characteristics in terms of projections of side-chains, flexibility and physiochemical properties, attributed to the variety of their sequences. Small cyclic and constrained peptides are popular as drug scaffolds in the pharmaceutical industry and our data highlight how amino acid side-chains can fine-tune conformations in these promising peptides.

PubMed: 27695949
DOI: 10.1007/s00726-016-2333-x

実験手法

SOLUTION NMR