2NB4

Solution structure of Q388A3 PDZ domain

2NB4 の概要

• エントリーDOI: 10.2210/pdb2nb4/pdb
• NMR情報: BMRB: 25961
• 分子名称: Putative uncharacterized protein (1 entity in total)
• 機能のキーワード: pdz nmr, unknown function
• 由来する生物種: Trypanosoma brucei brucei TREU927
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10985.44

構造登録者

Mei, S. (登録日: 2016-01-24, 公開日: 2016-02-24, 最終更新日: 2024-05-15)

主引用文献

Mei, S.,Dong, Y.,Zhang, J.,Zhang, X.,Tu, X.
Solution structure of Q388A3 PDZ domain from Trypanosoma brucei
J.Struct.Biol., 194:214-217, 2016

PubMed Abstract: PDZ domains are abundant protein interaction modules that often recognize short amino acid motifs at the C-termini of target proteins and regulate multiple biological processes. So far, no PDZ domain in Trypanosoma brucei, an eukaryotic parasite causing sleeping sickness, has been studied. Q388A3, conserved in the related kinetoplastid parasites, is a 1634-residue protein containing a PDZ domain at its C-terminus. In this work, the solution structure of Q388A3 PDZ domain was solved by NMR spectroscopy. Q388A3 PDZ domain adopts a PDZ-like fold composed by a five-stranded β-sheet capped by two α-helices, which is similar to the PDZ domains from HtrA family proteins. Meanwhile, Q388A3 PDZ domain shows some structural features quite different from HtrA PDZ domain.

PubMed: 26917351
DOI: 10.1016/j.jsb.2016.02.018

実験手法

SOLUTION NMR