2NAZ

The solution NMR structure of the C-terminal effector domain of BfmR from Acinetobacter baumannii

2NAZ の概要

• エントリーDOI: 10.2210/pdb2naz/pdb
• NMR情報: BMRB: 18849
• 分子名称: Transcriptional regulatory protein RstA (1 entity in total)
• 機能のキーワード: response regulator, effector domain, transcription regulator
• 由来する生物種: Acinetobacter baumannii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13689.29

構造登録者

Olson, A.L.,Thompson, R.J.,Cavanagh, J.,Feldmann, E.A.,Bobay, B.G. (登録日: 2016-01-15, 公開日: 2017-01-18, 最終更新日: 2024-05-15)

主引用文献

Draughn, G.L.,Milton, M.E.,Feldmann, E.A.,Bobay, B.G.,Roth, B.M.,Olson, A.L.,Thompson, R.J.,Actis, L.A.,Davies, C.,Cavanagh, J.
The Structure of the Biofilm-controlling Response Regulator BfmR from Acinetobacter baumannii Reveals Details of Its DNA-binding Mechanism.
J.Mol.Biol., 430:806-821, 2018

PubMed Abstract: The rise of drug-resistant bacterial infections coupled with decreasing antibiotic efficacy poses a significant challenge to global health care. Acinetobacter baumannii is an insidious, emerging bacterial pathogen responsible for severe nosocomial infections aided by its ability to form biofilms. The response regulator BfmR, from the BfmR/S two-component system, is the master regulator of biofilm initiation in A. baumannii and is a tractable therapeutic target. Here we present the structure of A. baumannii BfmR using a hybrid approach combining X-ray crystallography, nuclear magnetic resonance spectroscopy, chemical crosslinking mass spectrometry, and molecular modeling. We also show that BfmR binds the previously proposed bfmRS promoter sequence with moderate affinity. While BfmR shares many traits with other OmpR/PhoB family response regulators, some unusual properties were observed. Most importantly, we observe that when phosphorylated, BfmR binds this promoter sequence with a lower affinity than when not phosphorylated. All other OmpR/PhoB family members studied to date show an increase in DNA-binding affinity upon phosphorylation. Understanding the structural and biochemical mechanisms of BfmR will aid in the development of new antimicrobial therapies.

PubMed: 29438671
DOI: 10.1016/j.jmb.2018.02.002

実験手法

SOLUTION NMR