2N9V

Solution NMR Structure of the membrane localization domain from Pasteurella multocida toxin

2N9V の概要

• エントリーDOI: 10.2210/pdb2n9v/pdb
• 関連するPDBエントリー: 2N9W
• NMR情報: BMRB: 18565
• 分子名称: Dermonecrotic toxin (1 entity in total)
• 機能のキーワード: bacterial toxin, membrane localization domains, toxin
• 由来する生物種: Pasteurella multocida
• 細胞内の位置: Cytoplasm : P17452
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10532.07

構造登録者

Hisao, G.S.,Brothers, M.C.,Ho, M.,Wilson, B.A.,Rienstra, C.M. (登録日: 2015-12-12, 公開日: 2016-12-07, 最終更新日: 2024-05-15)

主引用文献

Hisao, G.S.,Brothers, M.C.,Ho, M.,Wilson, B.A.,Rienstra, C.M.
The membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution.
Protein Sci., 26:497-504, 2017

PubMed Abstract: Membrane localization domain (MLD) was first proposed for a 4-helix-bundle motif in the crystal structure of the C1 domain of Pasteurella multocida toxin (PMT). This structure motif is also found in the crystal structures of several clostridial glycosylating toxins (TcdA, TcdB, TcsL, and TcnA). The Ras/Rap1-specific endopeptidase (RRSP) module of the multifunctional autoprocessing repeats-in-toxins (MARTX) toxin produced by Vibrio vulnificus has sequence homology to the C1-C2 domains of PMT, including a putative MLD. We have determined the solution structure for the MLDs in PMT and in RRSP using solution state NMR. We conclude that the MLDs in these two toxins assume a 4-helix-bundle structure in solution.

PubMed: 27977897
DOI: 10.1002/pro.3097

実験手法

SOLUTION NMR